Fig. 2

NHERF1 and ERM-NF2 expression in normal and transformed CP provides new diagnostic markers for CP tumors. a IHC with NHERF1 and NF2 antibodies in normal CP shows apical plasma membrane and cytoplasmic distribution of NHERF1 and characteristic basolateral plasma membrane expression of NF2. The inset shows additional faint apical plasma membrane expression of NF2. The H& E panels in A–C are shown for comparison. b IHC with indicated antibodies of CP papilloma shows prominent apical plasma membrane expression of NHERF1 that is more pronounced than the Kir7.1 labeling, basolateral plasma membrane expression of NF2, strong ezrin expression in the cytoplasm and at the apical plasma membrane and moesin labeling of the apical plasma membrane but also of the vasculature. c IHC with NHERF1 and NF2 antibodies in CP carcinoma (Case CPC3, Additional File 1: Table S1) shows delocalization to the cytoplasm of NHERF1 and focally preserved basolateral expression of NF2 (inset). d Quantification of the NHERF1 subcellular distribution in normal CP, CP papilloma (CPP) and carcinoma (CPC) cases. PM, plasma membrane. *From 22 cases of CPP, one had predominant oncocytic-like morphology and was not included in this analysis