Fig. 7

Notch1 levels in the entorhinal cortex of AD patients. a) Representative Western Blot analysis on whole cell lysate indicates no difference in Notch1 levels, represented by the extracellular and the intracellular component, between healthy controls and AD patients. In contrast, p-Tau is increased in AD lysates. b–e) Western blot on synaptosomal fractions shows the unchanged expression of Notch1 in the synaptic (P2) component (p=0.2) of AD samples as compared to the controls (b and c). On the other hand, in the cytoplasmic compartment (S2) Notch1 levels are increased (p=0.007) in AD samples as compared to the controls (d and e). In addition, in both fractions, the levels of p-Tau are augmented in AD samples, (P2, p=0.02; S2, p=0.002) as compared to the age matched controls (c and e). f–g) Immunoblotting (f) and bar graph (g) on nuclear fraction indicates no difference in Notch1 expression between AD and healthy controls (p=0.25). *= p<0.05, **= p<0.01. Error bars are SEM