Fig. 2

Notch1 is expressed in A β42 positive plaques invaded by activated microglia and astroglia. a–a”) Fluorescent immunolabeling reveals that in AD patients, A β42 and Notch1 are present in core plaques (arrows in a’ and magnified insert) and fibrillary-like plaques (a”) with abundant activated microglia, CD68 positive. In (a’) a magnified insert shows a microglia (blue) ensheathed in an amyloid fibrillary Notch1 positive plaque. In the healthy controls (a), A β42 and CD68 immunoreactivity is significantly lower and Notch1 labels homogeneously the neuronal soma (insert). b–b”) Representative fluorescent immunolabeling shows that in AD patients, core plaques and fibrillary structures positive for Notch1 and A β42 are invaded by GFAP positive astroglia. GFAP immunoreactivity is significantly lower in healthy controls (b) as compared to the AD samples b’-b”). Scale bars in all panels are 40 μm