Fig. 5
From: Purkinje cell injury, structural plasticity and fusion in patients with Friedreich’s ataxia
An increase in bi-nucleate Purkinje cells is evident within the cerebellum of FRDA cases. a 3-dimensional confocal image of a bi-nucleate Purkinje cell immunofluorescently labelled with Calbindin-D28K (green) and DAPI nuclear stain (blue). The hatched area in (i) represents the higher magnified images (ii/iii). b A representative image of a bi-nucleate Purkinje cell containing two large Purkinje cell-like nuclei (Big/Big) and a bi-nucleate Purkinje cell with one large Purkinje cell-like nucleus and one smaller nucleus with compact chromatin (Big/Small). c Quantification of bi-nucleate Purkinje cells in FRDA cases. d Quantification of bi-nucleate Purkinje cells in FRDA cases categorised by their nuclear phenotype (Big/Big) or (Big/Small). Results are expressed as the mean +/- (SE) (**p < 0.01, comparing FRDA to control or Big/Big to Big/Small)