Fig. 7

Intracellular localization of TDP-43 (labeled in green) in NPC neuron-like cells. Cells were incubated for 24 h with either NAC 100 μM or CD 800 μM and TDP-43 localization was examined by immunofluorescence. a Untreated cells (NT). b NAC treatment. c CD treatment. d Quantitative evaluation of the percentage of neuron like cells displaying cytoplasmic localization of TDP-43 in NPC patients (n = 4) untreated or treated with NAC and CD. At least 400 cells have been counted for each cell line. Data are presented as mean ± SD of 3 independent experiments. **p < 0.01 in Student’s t test. Treatment with either NAC or CD determined a reduction of TDP-43 staining in the cytosolic compartment