Fig. 4

Immunofluorescence of phospho-TDP-43 (labeled in red) in wt (a) and NPC (b) neuron-like cells. TDP-43 immunostaining using a phospho-specific anti-TDP43 antibody showed the cytosolic accumulation of phosphor-TDP-43 in NPC neuronal-like cells. c-f Co-Immunofluorescence of TDP-43 (labeled in green) and the lysosomal marker LAMP-1 (labeled in red). g-j Co-Immunofluorescence of TDP-43 (labeled in green) and the autophagic marker p62 (labeled in red). Immunostaining showed that neither LAMP-1 or p62 co-localized with TDP-43. Nuclei were stained with DAPI (blue)