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Fig. 2 | Acta Neuropathologica Communications

Fig. 2

From: Critical residues involved in tau binding to fyn: implications for tau phosphorylation in Alzheimer’s disease

Fig. 2

Identification of PXXP motifs critical for tau binding to fyn-SH3. a Coomassie blue-stained gels of supernatants from cleared bacterial lysates following induction of protein expression (Binding S/N), show glutathione S transferase (GST) and GST-fyn-SH3 at the expected sizes of 27 and 36 kDa, respectively. Specific coupling of GST or GST-fyn-SH3 proteins is shown (Beads). Molecular weight markers (kDa) are shown on the left. b Western blots of GST-fyn-SH3 pull-downs from CHO cells expressing wild-type (WT) or mutant P → A tau probed with antibodies recognising tau (upper panel) or GST (middle panel). CHO cell lysates were probed with tau antibody for standardisation (lower panel). Lanes spliced from the same blot are indicated by a dotted line. Molecular weight markers (kDa) are shown on the left. c Quantification of the amount of tau immunoreactivity pulled down by GST-fyn-SH3, as a proportion of WT tau, following standardisation to total tau in the corresponding lysate. Values represent mean ± SEM, n = 6–22. One-way ANOVA with post-hoc Dunnett’s test, **p < 0.01, ***p < 0.001

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