Fig. 8

Correlative autoradiography (ARG) and immunohistochemical (IHC) findings for assessment of TDP-43 binding. (a-c) Case 2 - Normal, (d-i) Case 30 - FTLD-TDP. Shown from left to right AV-1451 ARG, AV-1451 Blocked ARG (BLK-ARG, shown with “letter”*), PHF-1 and TD-43 in the (a-f) posterior hippocampal and (g-i) superior temporal cortex. ARG images show no AV-1451 in the normal case (a-c) and minimal displaceable AV-1451 uptake in the (d) parahippocampal grey matter and (g) temporal lobe grey matter (red arrows) similar to regions of TDP-43 immunopositivity that is not seen in the normal brain (red dashed arrow, a). Minimal binding of AV-1451 is seen in the lateral geniculate nucleus (blue arrowheads) in both normal and TDP-43 positive cases is likely nonspecific. As an internal control, note that the grey matter parahippocampal AV-1451 signal in the normal case is absent (red dashed arrow) and the lateral geniculate (a) has greater signal—unlike the TDP case (d). Black arrowheads indicate 20x inset locations