Fig. 4

Correlative autoradiography (ARG) and immunohistochemical (IHC) findings for assessment of Pick’s disease (PiD, 3R Tau) binding. a, b Case 6 - AD for comparison (HpSp)/DLBD, c, d Case 14 - PiD, e-h Case 15 - PiD, i-l Case 16 – PiD. Shown from left to right AV-1451 ARG (AV-1451 blocking shown in insets) and PHF-1 in the a-f temporal cortex, g-j frontal cortex, and k-l parietal cortex for comparison. Moderate-severe AV-1451 displaceable binding in the AD case is shown (green arrows) corresponding with PHF-1. AV-1451 ARG displaceable signal is minimal and much weaker in the Pick’s cases than in the AD subject even in those with similar intensity of PHF-1 immunopositivity (i-l) to AD (red arrows). In some cases, loss of white matter-grey matter AV-1451 contrast may be the most notable feature of AV-1451 binding as compared to AV-1451 blocking studies (g, i). Scale bar for all full size cortical sections at 5 mm, and for 20x zoomed insets at 25 μm. Black arrowheads indicate 20x inset locations