Fig. 5
From: ALS-linked misfolded SOD1 species have divergent impacts on mitochondria
AMF7-63 and B8H10 antibodies detect aggregated misfolded SOD1 in spinal cords. a Size exclusion filter-trap assay of homogenates or isolated mitochondria from spinal cords of pre-symptomatic (10Â week) and early symptomatic SOD1G93A rats or age-matched SOD1WT controls, blotted for misfolded SOD1-specific antibodies B8H10, DSE2-3H1, AMF7-63 and C4F6. Data is representative of three independent trials. b Homogenates and isolated mitochondria immunoblotted for SOD1 to verify expression levels. Actin and VDAC serve as loading controls