Fig. 4

Immunogold electron microscopy for the detection of 53BP1 (a, b) and WRAP53 (c) in PDDF. 45d post-IR. Immunogold particles of 53BP1 immunoreactivity strongly decorated the network of chromatin fibers throughout the focus. c Similarly, immunogold labeling for WRAP53 was also detected on the fibers. Scale bar: a = 175 nm, b = 150 nm, c = 125 nm