Fig. 1

Fasudil treatment significantly reduces synT aggregation in H4 cells in vitro. a H4 neuroglioma cells were seeded in the presence or absence of Fasudil and 24 h later transfected with plasmids encoding for synT and SYPH1. 24 h after transfection, cultures were investigated by immunocytochemistry (ICC), Western blot (WB) and size-exclusion-chromatography-HPLC (SEC-HPLC). b ICC of synT and SYPH1 in H4 cells 24 h after transfection, treated with different Fasudil concentrations. Scale bar: 50 μm. c Quantification of transfected H4 cells with inclusions 24 h after transfection and treatment with different Fasudil concentrations. **p < 0.01, ANOVA with Dunnett post-hoc test, n = 4. d WB analysis of H4 protein lysates 24 h after transfection treated with different Fasudil concentrations revealed no significant differences in protein levels between groups. e Quantification of dot-blot analysis against α-Syn after SEC-HPLC fractionation of H4 protein lysates 24 h after Fasudil treatment. Fractions were grouped according to gel filtration standard components. Fraction 1: >670 kDa, fraction 2: 670-158 kDa, fraction 3: 158-17 kDa, fraction 4: <17 kDa. Data are given as mean ± SEM. *p < 0.05, T-Test, n(ctr): 8; n(Fas20): 6