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Fig. 2 | Acta Neuropathologica Communications

Fig. 2

From: Clobetasol promotes remyelination in a mouse model of neuromyelitis optica

Fig. 2

Clobetasol promotes remyelination in cerebellar slice cultures treated with AQP4-IgG and human complement. a. Mouse cerebellar slices were cultured for 7 days and then exposed for 20 h to AQP4-IgG (or control human IgG) and human complement (HC). Treatment with clobetasol (or vehicle control) was then started and slices were fixed for immunostaining 6 days later. b. Representative fluorescence images of control (non-NMO IgG) and AQP4-IgG treated cerebellar slices (both with human complement) showing immunostaining of astrocyte markers AQP4 and GFAP, myelin protein MBP, neuronal axon marker NF, microglial marker Iba-1, and activated complement C5b-9. c. AQP4, MBP and NF immunofluorescence in cerebellar slice cultures treated with AQP4-IgG and human complement, without or with clobetasol (left). Percentage loss of MBP immunofluorescence (mean ± S.E., 3 slices, ** P < 0.01) (right). d. High magnification confocal microscopy showing immunofluorescence of MBP and CC1 overlayed (left), with quantitation of CC1-positive cells per field (right)

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