Evaluation of alpha-synuclein immunohistochemical methods for the detection of Lewy-type synucleinopathy in gastrointestinal biopsies

Table 3 Intraclass correlation coefficient (ICC) for judge ratings of staining densities, for each testing laboratory (DM, TB, JK) and the central laboratory (AGC/FL), of each staining morphology type (LP = lamina propria granular pattern; EC = epithelial cell nuclear pattern; LG = lacy-granular pattern; PV = perivascular-vascular pattern)

	Box 1 DM-LP	Box 2 TB-EC	Box 2 TB-LP	Box 3 JK-LG	Box 3 JK-LG^a	Box 4 AGC/FL-EC	Box 4 AGC/FL-LP	Box 4 AGC/FL-PV
n	12	12	12	12	11	12	12	12
Four Raters
ICC	0.73	−0.04	0.02	0.81	0.78	NA	0.53	NA
Three Raters
ICC	0.88	−0.05	0.00	0.79	0.72	0.16	0.78	0.80

Some judges did not rate some staining patterns for some laboratories; results are therefore given for four judges where all judge scores were available and for the three common judges when one or more judge scores were missing. Cases without submucosa in the slide were excluded from results for staining morphologies dependent on the presence of submucosa (Box 3, JK-LG)
^aExcluding insufficient submucosa

ISSN: 2051-5960