Primary antibody | Epitope exposure | Signal development | |
---|---|---|---|
AGC/FL (Angers, France) | p-synuclein, #64 WAKO, Osaka 1:30,000 | Formic acid (80 %) 20 min | BOND MAX Leica Biosystems, Newcastle Upon Tyne, U.K. |
TB (Sun City, AZ) | p-synuclein, polyclonal H. Akiyama, Tokyo 1:10,000 | Proteinase K 1:100 37 ° C, 20 min | ABC, DAB Vector Laboratories |
JK (Chicago, IL) | Alpha-synuclein, LB509 Invitrogen 1:500 | Formic acid (88 %) 20 min | ABC, DAB Vector Laboratories |
DM (Toronto, Canada) | p-synuclein, #64 WAKO, Osaka 1:12,000 | Ventana Protease 2 72 ° C, 8 min Citrate buffer, pH 6 4 90 ° C, 4 min | Ventana Benchmark Ultra, Optiview multimer and amplifier |