Fig. 5

Pre- and post- synaptic protein amounts are altered during AD development. Representative blots of (a) supernatants and (b) pellets from cortical homogenates of postmortem brain. Blots were probed with antibodies against synapsin I holoprotein (74 kDa), the NR2B subunit of N-methyl D-aspartate receptor (NR2B,170 kDa) and post-synaptic density 95 protein (PSD95, 95 kDa. Blots were also probed with antibodies against neuron-specific enolase (NSE, 45 kDa) or β-actin (42 kDa) as loading controls. Bar graphs show amounts of synapsin I, NR2B and PSD95, all normalised to their respective loading control. CTRL: control (n = 5), Braak II AD (n = 4), Braak III AD (n = 3), Braak IV AD (n = 4), Braak V AD (n = 3), Braak VI AD (n = 5). Data is mean ± SEM. *p < 0.05, **p < 0.01