Fig. 6

The relationship between the distribution of αSyn or PαSyn and mitochondrial membrane proteins. Upper panels, Parkinson’s disease (PD); a-e Dorsal motor nuclei of the vagus; f Locus coeruleus; a-c Double immunohistochemistry for αSyn (blue) and CCO (brown); d, e Double immunohistochemistry for PαSyn (brown) and TOM20 (blue); f Double immunohistochemistry for αSyn (blue) and TOM20 (brown). Lower panels, PLA2G6-associated neurodegeneration (PLAN); a-d Dorsal motor nuclei of vagus; e-h Locus coeruleus; i Substantia nigra; a-c Double immunohistochemistry for αSyn (blue) and CCO (brown); d, i Double immunohistochemistry for αSyn (blue) and TOM20 (brown); e-h Double immunohistochemistry for PαSyn (brown) and TOM20 (blue). Scale bar represents 8 μm in the upper panel and (a-f) in the lower panel (a-i). Upper panel, Parkinson’s disease (PD). a There is an amorphous deposit of αSyn (arrow) in the CCO-positive cytoplasm. b In the center of the αSyn-positive LB (arrow), the expression of CCO is negative. c The sausage-like Lewy body (LB) has a small rim, which is CCO-positive (arrow). d There are a few PαSyn-positive granules (arrow) that are mildly TOM20-positive on the surface. e The neuron is filled with sausage-like LBs arrows, which are almost negative for TOM20. f In the neuron, the surface of the LB is mildly positive for TOM20. Lower panel, PLA2G6-associated neurodegeneration (PLAN) a There are some CCO-positive dots around the irregular αSyn-positive deposit (arrow). b αSyn-positive inclusion (arrow) contains some CCO-positive granules. c CCO expression is seen in only a part of the surface (arrows) of αSyn-positive LB. d Under the αSyn deposit, there are clear TOM20-positive dots (arrow) in the cytoplasm. e In the neuronal cytoplasm, there are several PαSyn-positive granules (arrow), on which the surface is completely TOM20-positive. f PαSyn-positive granules are clustered to form a thin inclusion (arrow), on which the surface is positive for TOM20. g PαSyn-positive granules with TOM20-positive rim are clustered to form one round inclusion (arrow). h In the core of the LB, the immunoreaction of PαSyn and TOM20 are absent. In the halo of LB (arrow), PαSyn-positive granules with a TOM20-positive rim make a line. i PαSyn and TOM20-positive membrane-like structure covers the surface of LB (arrow)