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Fig. 3 | Acta Neuropathologica Communications

Fig. 3

From: High expression of α-synuclein in damaged mitochondria with PLA2G6 dysfunction

Fig. 3

Age-dependent morphological changes in PαSyn-positive mitochondria in axons. a-f paraffin section; g, h epon semi-thin section; i-m ultramicroscopy; a, g-j Pla2g6-KO mouse at 15 weeks; (b, c) Pla2g6-KO mice at 1 year; d-f, k-m, Pla2g6-KO mice at the 2 years. a, b, e, g, h, j, l, m Anterior column of the spinal cord; c, f anterior root; d, i, k perinuclear of neuron; a, b, d, e Immunohistochemistry for PαSyn; c, f double immunohistochemistry for PαSyn (blue) and TOM20 (brown); g thionine staining; h PAS staining. Scale bars represent 40 μm in (a, b, e), 10 μm in (c, d, g, h), 5 μm in (f), 500 nm in (i and k), and 1 μm in (j, l, m). a At preclinical-stage, small PαSyn-positive granules (arrows) could be seen in axons. b At early symptomatic stage, the PαSyn-positive granules became large and irregular-shaped (arrows). c Co-localization of PαSyn and TOM20 is evident (arrows) in the granules. d At end stage, PαSyn-positive granules and membranes (arrow) are seen in neurons and in the neuropil. e PαSyn-positive membranous structures (arrows) coincide with the small PαSyn-positive granules. f The membranous structures and granules that are positive for PαSyn and TOM20 combine into a complex (arrows). g, h In the axons, there are many dark-colored granules (arrows in g), which are clearly stained by PAS (arrow in h). i In the perinuclear space of swollen neuron filled with granules, mitochondrion with partly degenerated crista (arrowhead) and numerous round bodies filled with dense granules. j In a myelinated axon, many spherical mitochondria and round bodies filled with dense granules are seen. The structure of the axon is virtually normal. k In the mitochondrion (arrowhead) around the nuclei of neurons, most of the cristae are replaced by dense granules. l Many mitochondria with tubular and vesicular cristae (arrowheads) cluster in the axon. m Membranous degeneration, tubulovesicular structures (black arrowhead) and abnormal mitochondria (arrowhead) can be seen together in the spheroid

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