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Fig. 1 | Acta Neuropathologica Communications

Fig. 1

From: Super-resolution microscopy reveals γ-secretase at both sides of the neuronal synapse

Fig. 1

Localization of γ-secretase in axons, dendrites and spines of mouse primary hippocampal neurons analyzed by confocal and STED microscopy as well as dSTORM. γ-Secretase was stained with GTB in combination with streptavidin conjugated with either Alexa594 (confocal and STED microscopy) or Alexa647 (confocal and dSTORM). (a) Confocal image of a zoomed dendrite stained for MAP2 (blue), surrounding axons stained for tau (green) and γ-secretase (red). The individual stains are displayed in the upper panel, while different combinations of merged images are shown in the lower panel, as indicated. (b) Confocal image of PS1 and γ-secretase labeling in a close-up of a dendrite. Arrows point at synaptic regions with co-staining of PS1 and γ-secretase (yellow). (c) Confocal image of PS1 and nicastrin labeling in a close-up of a dendrite. Arrows point at synaptic regions with co-staining of nicastrin and γ-secretase (yellow). (d) Confocal image of an axon from the same cell as in (a) with tau staining in green in the upper panel, γ-secretase in red in the middle panel and a merged image in the bottom panel. The inset shows a zoomed portion of the merged image with the addition of MAP2 (blue). (e) A combination of confocal (for tau, upper panel) and STED microscopy (for γ-secretase, middle panel) was used to visualize the enrichment of active γ-secretase in the axonal boutons. A merged image is shown in the bottom panel. (f) dSTORM image of active γ-secretase (red) overlayed with a brightfield image. Note enrichment of active γ-secretase at spines

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