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Fig. 1 | Acta Neuropathologica Communications

Fig. 1

From: X-linked intellectual disability gene CASK regulates postnatal brain growth in a non-cell autonomous manner

Fig. 1

CASK deletion from Purkinje cells or granule cells does not alter cerebellar development. a Representative brain sections from two months old control mice carrying a floxed CASK gene (XflCASK/Y) and mice with CASK knocked out from Purkinje cells (XflCASK/Y; PCP2-cre); sections are stained with green (nucleic acid stain) and red (calbindin). Arrows indicate Purkinje cells. b Quantitation of molecular layer thickness and (c) Quantitation of Purkinje cell diameter; data are plotted as mean ± SEM, n = 3 mice. d, e, f Locomotion of mice analyzed in home cage using an overnight force plate experiment indicating no obvious change in locomotion. Average speed, average highest speed and average % activity are quantified and data are plotted as mean ± SEM, n = 3 mice. g Quantitation of retention time of mice on fixed speed rotarod, data are plotted as mean ± SEM, n = 4 mice. Floxed indicates CASK floxed mice and PCKO indicates Purkinje cell-specific CASK knockout mice. h Representative brain sections from two month old mice with indicated genotypes showing that CASK deletion from cerebellar granule cells does not lead to neuronal death or alteration in layering. LSL-tdTomato is a Cre indicator line in which a loxP-flanked stop cassette prevents expression of a red fluorescent protein; Math5-Cre mouse line has Cre recombinase expressed under the Math5 promoter. Arrows indicate inner granular layer where Cre-recombinase is expressed. Please note that the top two panels are cerebellar sections from mice with unperturbed CASK gene. i Representative brain images of two month old mice with indicated genotype. j Parasaggital sections of cerebellum showing that CASK deletion from granule cells does not lead to cerebellar hypoplasia. k Quantitation of cerebellar area in parasagittal sections; data are plotted as mean ± SEM, n = 3. Floxed indicates CASK floxed mice and GCKO indicates granule cell-specific CASK knockout mice

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