Fig. 5

Ablation of CCR2⁺Ly6C^hi monocytes increases Aβ accumulation in T. gondii infected 5xFAD mice. T. gondii infected 5xFAD mice were treated with the anti-CCR2 monoclonal antibody MC-21 to specifically ablate CCR2⁺Ly6C^hi monocytes. a, b Ly6C^hi monocytes in the blood were measured 28 days post infection and after 13 days of anti-CCR2 administration. Representative dot plots picture the gating strategy and the specific ablation of Ly6C^hi monocytes in (b) anti-CCR2 treated animals compared to (a) PBS treated animals. c, d Ly6C^hi monocytes in the blood on d22 and d28 as well as in the brain on d28 were analyzed and their percentage of live cells is displayed. e The amount of Aβ₄₂ in the brains of 5xFAD mice after T. gondii infection with and without monocyte ablation was measured by ELISA. Data (n = 4 per group) are presented as mean + SEM. Significance levels (p values) determined by unpaired Student’s t test are indicated. *p ≤ 0.05, **p ≤ 0.01