Fig. 4

Recruited mononuclear cells are potent Aβ phagocytic cells. a–c Ex vivo phagocytosis assay was performed with mononuclear cells isolated from 5xFAD and C57BL/6 mouse brains and cleared from dead cells by sorting via flow cytometer. a Populations were gated as described in Fig. 3a. Representative histograms show the uptake of fluorescence labeled Aβ₄₂ peptide by different cell populations. Gray curves show the 4 °C control (dark gray) and the Aβ₄₂ ⁻ control (light gray) for each population. b Bars indicate the median fluorescence intensity (MFI) of each population to express differences in the amount of Aβ₄₂ taken up. Data are displayed as mean + SEM (n = 4-5). c Representative images obtained with FlowSight™ are shown for each population. Scale bar, 20 μm. Significance levels (p values) determined by Fisher’s LSD test are indicated. ns, not significant, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001