Fig. 1From: Chronic Toxoplasma gondii infection enhances β-amyloid phagocytosis and clearance by recruited monocytes T. gondii infection leads to reduced plaque burden in 5xFAD mice. a, a’ show representative cortex regions with immunohistochemical labeling against Aβ from (a) non-infected and (a’) T. gondii infected 5xFAD mice. Arrow heads point towards remaining plaques in infected animals. Scale bars represent 1000 μm (left) and 200 μm (right). b Quantification of cortical Aβ plaques. Data is displayed as mean ± SEM. Significance as determined by unpaired t test with Welsh‘s correction is indicated. **p ≤ 0.01. c, c’ Ex vivo two-photon micrographs of the cerebral cortex layers I and II of (c) control and (c’) T. gondii infected 5xFAD mice injected with Methoxy-X04. Amyloid plaques stained with Methoxy-X04 are represented in green, autofluorescence is shown in red. One representative z-stack projection spanning at least 50 μm depth is shown for each condition. Scale bar, 100 μm. d Plaque volume in cortical layers I and II determined from at least five ex vivo two-photon micrographs shown in (c, c’). Each symbol represents one plaque and bars represent the mean, statistical significance is denoted by asterisks. **p ≤ 0.01Back to article page