Fig. 1

T. gondii infection leads to reduced plaque burden in 5xFAD mice. a, a’ show representative cortex regions with immunohistochemical labeling against Aβ from (a) non-infected and (a’) T. gondii infected 5xFAD mice. Arrow heads point towards remaining plaques in infected animals. Scale bars represent 1000 μm (left) and 200 μm (right). b Quantification of cortical Aβ plaques. Data is displayed as mean ± SEM. Significance as determined by unpaired t test with Welsh‘s correction is indicated. **p ≤ 0.01. c, c’ Ex vivo two-photon micrographs of the cerebral cortex layers I and II of (c) control and (c’) T. gondii infected 5xFAD mice injected with Methoxy-X04. Amyloid plaques stained with Methoxy-X04 are represented in green, autofluorescence is shown in red. One representative z-stack projection spanning at least 50 μm depth is shown for each condition. Scale bar, 100 μm. d Plaque volume in cortical layers I and II determined from at least five ex vivo two-photon micrographs shown in (c, c’). Each symbol represents one plaque and bars represent the mean, statistical significance is denoted by asterisks. **p ≤ 0.01