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Fig. 3 | Acta Neuropathologica Communications

Fig. 3

From: Retention of hexanucleotide repeat-containing intron in C9orf72 mRNA: implications for the pathogenesis of ALS/FTD

Fig. 3

Nuclear accumulation of intron 1-retaining C9orf72 transcripts. Partitioning of intron 1-retaining and correctly spliced C9orf72 polyadenylated transcripts was compared between nuclear and cytoplasmic fractions from lymphoblasts from G4C2 repeat expansion carriers and controls. a Poly(A)+ RNA was analyzed in nuclear and cytoplasmic fractions by RT-PCR as in Fig. 1. The nuclear long non-coding RNA, NEAT1, was used to confirm the purity of the fractions. GAPDH was used as a loading control. b Levels of nuclear and cytoplasmic unspliced and spliced C9orf72 transcripts were compared according to cell equivalent and the ratios nuclear/(nuclear + cytoplasmic) were calculated for each product. Data represent mean ± SEM, n = 6 (C9+); 7 (C9), ** P < 0.01, *** P < 0.001. C9, controls; C9+, expansion carriers

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