Fig. 6
Reduction of ErbB4 expression precedes loss of NRG1 puncta in the lumbar motor neurons of SOD1G93A mice. (a–d) The lumbar spinal cord sections from 5 months old wild-type mice were stained with the antibodies for ErbB4, VAChT, and NRG1. The representative images for motor neurons stained with NRG1/ErbB4 (a) or VAChT/ErbB4 (c) with corresponding magnified Z axis-images of the C-bouton (b, d, arrows). Bars: 10 μm. (e–h) The lumbar spinal cord sections from 5 months old wild-type (WT; e) or SOD1G93A mice at 3 (f), 3.5 (g) and 5 months old (h) were stained with the antibodies for ErbB4, VAChT, and NRG1. Representative images in the anterior horn area were shown with the merged images. Arrows indicate the motor neurons surrounded by VAChT- and NRG1-positive puncta showed diminished expressions of ErbB4. Bars: 50 μm