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Fig. 2 | Acta Neuropathologica Communications

Fig. 2

From: Neuregulin 1 confers neuroprotection in SOD1-linked amyotrophic lateral sclerosis mice via restoration of C-boutons of spinal motor neurons

Fig. 2

Age-dependent losses of NRG1 in the lumbar spinal cord of SOD1G93A and SOD1G85R mice. (aā€“l) Representative images of the lumbar spinal cord of 4Ā month old wild-type (WT) mice (aā€“c), SOD1G93A (G93A) mice (dā€“f), 12Ā month old wild-type (WT) mice (gā€“i), and SOD1G85R (G85R) mice (jā€“l) stained with the antibodies for NRG1 (a, d, g, j), VAChT (b, e, h, k), and NRG1/VAChT/NeuN (c, f, i, l). Bar: 50Ā Ī¼m (m, n) Quantification of the number of NRG1-positive puncta per motor neuron in wild-type and SOD1G93A mice (m) or wild-type and SOD1G85R mice (n) at indicated ages. ES denotes endstage. At least 30 (SOD1G93A) or 60 (SOD1G85R) motor neurons from mutant SOD1 (nā€‰=ā€‰3āˆ’5) or wild-type mice (nā€‰=ā€‰2āˆ’3) were analyzed for NRG1-positive puncta. Fewer number of motor neurons were analyzed in symptomatic SOD1G93A mice due to loss of well-defined shaped motor neurons. Red bars represent average from both groups. ***: pā€‰<ā€‰0.001, ****: pā€‰<ā€‰0.0001 (Dunnā€™s Multiple Comparison test (m) or Bonferroniā€™s Multiple Comparison test (n)). (o, p) mRNA levels of NRG1 type III (o) and type I (p) in the lumbar spinal cords of wild-type, SOD1G93A, and SOD1G85R mice at indicated ages. The mean mRNA levels of NRG1 isoforms relative to the ones of wild-type mice at 3Ā month old (left) or 6Ā month old (right) obtained by quantitative PCR analysis were plotted. *: p <0.05, ***: p <0.001 (Tukeyā€™s multiple comparison test). Error bars denote SD

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