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Fig. 2 | Acta Neuropathologica Communications

Fig. 2

From: Neuregulin 1 confers neuroprotection in SOD1-linked amyotrophic lateral sclerosis mice via restoration of C-boutons of spinal motor neurons

Fig. 2

Age-dependent losses of NRG1 in the lumbar spinal cord of SOD1G93A and SOD1G85R mice. (al) Representative images of the lumbar spinal cord of 4 month old wild-type (WT) mice (ac), SOD1G93A (G93A) mice (df), 12 month old wild-type (WT) mice (gi), and SOD1G85R (G85R) mice (jl) stained with the antibodies for NRG1 (a, d, g, j), VAChT (b, e, h, k), and NRG1/VAChT/NeuN (c, f, i, l). Bar: 50 μm (m, n) Quantification of the number of NRG1-positive puncta per motor neuron in wild-type and SOD1G93A mice (m) or wild-type and SOD1G85R mice (n) at indicated ages. ES denotes endstage. At least 30 (SOD1G93A) or 60 (SOD1G85R) motor neurons from mutant SOD1 (n = 3−5) or wild-type mice (n = 2−3) were analyzed for NRG1-positive puncta. Fewer number of motor neurons were analyzed in symptomatic SOD1G93A mice due to loss of well-defined shaped motor neurons. Red bars represent average from both groups. ***: p < 0.001, ****: p < 0.0001 (Dunn’s Multiple Comparison test (m) or Bonferroni’s Multiple Comparison test (n)). (o, p) mRNA levels of NRG1 type III (o) and type I (p) in the lumbar spinal cords of wild-type, SOD1G93A, and SOD1G85R mice at indicated ages. The mean mRNA levels of NRG1 isoforms relative to the ones of wild-type mice at 3 month old (left) or 6 month old (right) obtained by quantitative PCR analysis were plotted. *: p <0.05, ***: p <0.001 (Tukey’s multiple comparison test). Error bars denote SD

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