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Fig. 1 | Acta Neuropathologica Communications

Fig. 1

From: Neuregulin 1 confers neuroprotection in SOD1-linked amyotrophic lateral sclerosis mice via restoration of C-boutons of spinal motor neurons

Fig. 1

Neuregulin-1 is localized in the post-synaptic face of C-bouton in the mouse spinal motor neurons. (a) Domain structures for murine Neuregulin-1 (NRG1) type III (Type III-β1a, based on gene accession number: NM 178591) and type I (Type I-β1a, based on gene accession number: AY648976). The anti-NRG1 antibody used in this study recognizes both Type I and Type III NRG1. Arrows indicate the sites of primers used for quantitative PCR in Fig. 2. (bd) Representative images of lumbar spinal cord sections from 4 month old wild-type mice (C57BL/6) stained with the antibodies for neuregulin-1 (NRG1) (b), Kv2.1 (c), along with the merged image stained by NRG-1, Kv2.1, and choline acetyltransferase (ChAT) antibodies (d). Inset showed magnified image of C-bouton indicated by asterisk (d). (eh) Representative images of lumbar spinal cord sections from 4 month old wild-type mice stained with the antibodies for neuregulin-1 (NRG1) (e), m2 muscarinic acetylcholine receptor (m2) (f), along with the merged image stained with NRG1, m2, and ChAT antibodies (g). Magnified Z-axis images of the C-bouton in (eg: arrow) (h). (i) Representative image of motor neuron stained by NRG1, VAChT, and NeuN antibodies. (j) Magnified Z axis-images of the C-bouton in (e: asterisk). Bar: 20 μm (bd, i), 12.5 μm (eg)

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