Fig. 2
Banding patterns of PrPres in the brain and spleen tissue of ovine PrP mice infected with MM2-sCJD prions. (a) PrPres banding patterns in the brain and spleen tissue of ovine PrP mice (tg338 line) over two serial passages of MM2-sCJD (MM2). Note the different patterns in brain and spleen, which were designated T2Ov (red arrow) and T1Ov (blue arrow), respectively. These patterns can be compared with those observed after inoculation of 127S scrapie prions, a strain with a 21 kDa signature in brain and spleen [34]. (b) Immunoblots showing PrPres pattern in the brain and spleen of tg338 mice over serial passage of uncloned MM2-sCJD (MM2) and cloned (Cl.) MM2-sCJD prions. Note the low levels of T2Ov PrPres in the spleens of tg338 mice inoculated with cloned MM2-sCJD prions, which are only visible on overexposed gels. The equivalent of 1 and 2 mg of tg338 brain and spleen tissue was loaded on the SDS-PAGE gels. The blots were probed with Sha31 antibody