Fig. 4

APP/PSEN1 and Sorcs1 -/- x APP/PSEN1 have similar levels of Aβ as well as plaque load within the brain. Hemibrains of male and female APP/PSEN1 (n = 5–12/group) and Sorcs1 -/- x APP/PSEN1 (n = 10/group) mice were processed via differential detergent solubilization to produce fractions of TBS-soluble, Triton-X-soluble and formic-acid soluble Aβ. Levels of Aβ 40 (a-f) and Aβ 42 (g-l) were determined from each fraction via ELISA. Total Aβ equals the sum of Aβ from each fraction. The Aβ42/40 ratio was calculated for each fraction (m-r). Oligomeric Aβ was assessed from the TBS-soluble fraction via dotblot analyses using OC (s, v), A11 (t, w) and NU-4 (u, x) antibodies. 6E10 positive Aβ plaque load was assessed via immunohistochemistry within the cortex and hippocampus (y) of male and female APP/PSEN1 and Sorcs1 -/- x APP/PSEN1 mice (n = 2-3/group). a - x P > 0.05, Student’s t-tests. Data expressed as mean ± s.e.m. y scale bar = 1 mm