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Table 3 Semiquantitative evaluation of signal intensities in immunolocalization studies

From: New insights into the protein aggregation pathology in myotilinopathy by combined proteomic and immunolocalization analyses

Protein A1 A2 A3
Z-disc (−associated) proteins
 Myotilin ↑↑↑ ↓∕-
 Filamin C ↑↑∕↑↑↑ ↑↑↑ ↑↑∕↑↑↑
 N-RAP ↑↑ ↑↑↑ ↑↑
 XIRP2 ↑↑∕↑↑↑ ↑↑↑
 Xin ↑↑∕↑↑↑ ↑↑↑ (↑)
 Obscurin ↑↑ ↑↑↑
 Myopalladin ↑↑∕↑↑↑ ↑↑↑ ↑∕↑↑
 Desmin ↑↑↑ (↑) ↑↑↑
 Plectin ↑↑↑
 Nestin ↑∕↑↑ - ↑↑↑
 Syncoilin ↑∕↑↑ ↑↑↑ ↑∕↑↑
Chaperone-assisted selective autophagy (CASA)/NBR1-mediated selective autophagy
 BAG3 ↑↑∕↑↑↑ - ↑↑↑
 HSPB8/Hsp22 ↑↑ ∕ ↑↑↑ ↑↑↑ ↑↑↑
 HSPA8/Hsc70 ↑↑ ↑↑↑
 p62 ↑↑↑ (↑)∕↑↑ (↑)
 NBR1 ↑↑∕↑↑↑ ↑↑∕↑↑↑
Further chaperones
 αB-crystallin ↑∕↑↑ ↑↑↑
 HSPB1/Hsp27 ↑∕↑↑ ↑↑↑ ↑↑↑
 Hsp70 ↑↑ ↑↑↑
Unfolded protein response (UPR) / oxidative stress response/transcription regulation
 BiP ↑↑ ↑↑ (↑)
 Thrombospondin 4 ↑↑∕↑↑↑ ↑↑↑ ↑↑
 MGST3 ↑↑↑ - ↑↑↑
 YBX3 ↑ ∕ ↑↑
Actin dynamics
 MURC (↑) ∕ ↑↑ (↑) ↑↑↑
 MYBPH (↑) ∕ ↑↑↑ ↑↑↑ (↑)
 Tropomodulin 1 ↑↑ ∕ ↑↑↑ ↑↑↑ (↑)
 CSRP3 ↑↑
Microtubule organization / vesicle transport
 α-tubulin ↑↑ ↑↑↑
 MAP4 ↑↑ ∕ ↑↑↑ (↑) ↑↑↑
 PACSIN3 (↑) ∕ ↑↑ - ↑↑↑
Sarcolemmal proteins
 Dystrophin ↑↑ - ↑↑ ∕ ↑↑↑
 Dysferlin ↑↑ ∕ ↑↑↑ ↑↑ ∕ ↑↑↑
 Supervillin ↑ ∕ ↑↑ - ↑↑↑
 δ-Sarcoglycan (↑) (↑) (↑)
  1. A1: abnormal area 1, A2: abnormal area 2, A3: abnormal area 3; (↑)∕↑∕↑↑∕↑↑↑: slightly/moderately/distinctly/strongly increased immunoreactivity compared to control fibers; ↓: decreased immunoreactivity compared to control fibers; −: no visible immunoreactivity