Table 2 Antibodies used in immunohostochemical stainings, their dilutions and supplier
From: Diagnostically important muscle pathology in DNAJB6 mutated LGMD1D
Antibody | Dilution | Supplier |
|---|---|---|
Myotilin | 1:50 | Leica Biosystems, Novocastra |
Alpha B crystallin | 1:10 | Leica Biosystems, Novocastra |
Desmin | 1:800 | Biogenex |
Dystrophin 2 (c-terminus) | 1:100 | Leica Biosystems, Novocastra |
SMI-31 | 1:1000 | Biosite |
SMI-310 | 1:400 | Abcam |
TDP-43 | 1:175 | Proteintech Europe |
P62 (SQSTM1) | 1:100 | Santa Cruz Biotechnology |
Ubiquitin | 1:300 | Dako |
LAMP2 | 1:50 | Southern Biotech |
LC3 | 1:50 | Novus Biologicals |
LC3B | 1:50 | Cell Signaling technology |
VCP | 1:600 | Thermo Scientific |
FHL-1 | 1:200 | Lifespan biosciences |
DNAJB6 | 1:100 | Abnova |
HSPB8 | 1:200 | Abcam |
BAG3 | 1:800 | Proteintech Europe |
Proteasome | 1:30 | Atlas antibodies |
Beta-amyloid | 1:30 | Leica Biosystems, Novocastra Life |
Amyloid precursor protein | 1:50 | Biosciences |
Wip1 | 1:50 | Sigma |
Dystrophin 1 (rod domain) | 1:40 | Leica Biosystems, Novocastra |
Dystrophin 2 (c-terminus) | 1:100 | Leica Biosystems, Novocastra |
Dystrophin 3 (N-terminus) | 1:20 | Leica Biosystems, Novocastra |
Dysferlin | 1:200 | Leica Biosystems, Novocastra |
α-Sarcoglycan | 1:100 | Leica Biosystems, Novocastra |
β-Sarcoglycan | 1:100 | Leica Biosystems, Novocastra |
γ-Sarcoglycan | 1:15 | Leica Biosystems, Novocastra |
α-Dystroglycan | 1:200 | Millipore, Upstate |
Caveolin 3 | 1:150 | Santa Cruz Biotechnology |
Merosin Laminin Alpha 2 Chain | 1:500 | USBiological |
Utrophin | 1:20 | Leica Biosystems, Novocastra |
Spectrin | 1:50 | Leica Biosystems, Novocastra |