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Table 2 Antibodies used in immunohostochemical stainings, their dilutions and supplier

From: Diagnostically important muscle pathology in DNAJB6 mutated LGMD1D

Antibody Dilution Supplier
Myotilin 1:50 Leica Biosystems, Novocastra
Alpha B crystallin 1:10 Leica Biosystems, Novocastra
Desmin 1:800 Biogenex
Dystrophin 2 (c-terminus) 1:100 Leica Biosystems, Novocastra
SMI-31 1:1000 Biosite
SMI-310 1:400 Abcam
TDP-43 1:175 Proteintech Europe
P62 (SQSTM1) 1:100 Santa Cruz Biotechnology
Ubiquitin 1:300 Dako
LAMP2 1:50 Southern Biotech
LC3 1:50 Novus Biologicals
LC3B 1:50 Cell Signaling technology
VCP 1:600 Thermo Scientific
FHL-1 1:200 Lifespan biosciences
DNAJB6 1:100 Abnova
HSPB8 1:200 Abcam
BAG3 1:800 Proteintech Europe
Proteasome 1:30 Atlas antibodies
Beta-amyloid 1:30 Leica Biosystems, Novocastra Life
Amyloid precursor protein 1:50 Biosciences
Wip1 1:50 Sigma
Dystrophin 1 (rod domain) 1:40 Leica Biosystems, Novocastra
Dystrophin 2 (c-terminus) 1:100 Leica Biosystems, Novocastra
Dystrophin 3 (N-terminus) 1:20 Leica Biosystems, Novocastra
Dysferlin 1:200 Leica Biosystems, Novocastra
α-Sarcoglycan 1:100 Leica Biosystems, Novocastra
β-Sarcoglycan 1:100 Leica Biosystems, Novocastra
γ-Sarcoglycan 1:15 Leica Biosystems, Novocastra
α-Dystroglycan 1:200 Millipore, Upstate
Caveolin 3 1:150 Santa Cruz Biotechnology
Merosin Laminin Alpha 2 Chain 1:500 USBiological
Utrophin 1:20 Leica Biosystems, Novocastra
Spectrin 1:50 Leica Biosystems, Novocastra