Fig. 5From: Low autophagy capacity implicated in motor system vulnerability to mutant superoxide dismutaseImpaired autophagy increases hSOD1G127X aggregation, and reduces the amount of soluble species throughout the disease course. The spinal cords (n = 5 per genotype per disease stage) were separated into three distinct fractions based on detergent solubility: whole homogenates, detergent-insoluble, and detergent-soluble fraction. a Western blots for hSOD1G127X protein in the three fractions. β-Actin in whole homogenates was used as a loading control. b-d Amounts of (b) total, (c) insoluble, and (d) soluble hSOD1G127X protein. *P < 0.05 vs. hSOD1G127X mice at 150 days of age. **P < 0.01 vs. disease stage-matched hSOD1G127X mice (one-way ANOVA with Tukey-Kramer’s test). e Upper: Filter-trapped hSOD1G127X aggregates in the spinal cords of the mice (n = 5 per genotype per disease stage). Lower: Amounts of hSOD1G127X aggregates. *P < 0.05 vs. hSOD1G127X mice at 150 days of age. **P < 0.01 vs. disease stage-matched hSOD1G127X mice (one-way ANOVA with Tukey-Kramer’s test). f Immunohistochemistry for hSOD1G127X protein in lumbar spinal cords of mice. Scale bars: 100 μmBack to article page