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Table 3 Antibodies used for immunohistochemistry

From: Small heat shock proteins are induced during multiple sclerosis lesion development in white but not grey matter

Antigen Clone Species Isotype Dilution Antigen retrieval Manufacturer
PLP Plpc1 Mouse IgG2a 1:3000 none AbD Serotec
HLA-DR LN3 Mouse IgG2b 1:1000 Citrate buffer eBioscience
Olig2 Polyclonal Rabbit   1:200 Citrate buffer Millipore
GFAP 6 F2 Mouse IgG1 1:200 Citrate buffer Monosan
HSPB1 EPR7278 Rabbit IgG 1:500 Citrate buffer Abcam
HSPB5 JAM01 Mouse IgG1 1:100 Citrate buffer In house
HSPB6 EPR14458 Rabbit IgG 1:500 Citrate buffer Abcam
HSPB8 Polyclonal Rabbit   1:500 Citrate buffer Abcam
HSPB11 polyclonal Rabbit   1:50 Citrate buffer (20 min) Novus
  1. PLP proteolipid protein, HLA-DR human leukocyte antigen, GFAP glial fibrillary acid protein, HSPB heat shock protein B