Table 2 Primer sequences
Markers | GeneID | Sequence forward | Sequence reverse | Size (bp) | Annealing temperature (°C) |
|---|---|---|---|---|---|
HSPB1 | 3315 | ACGGTCAAGACCAAGGATGG | AGCGTGTATTTCCGCGTGA | 104 | 58 |
HSPB6 | 126393 | TGCTAGACGTGAAGCACTTCT | ACCACCTTGACAGCAATTTCC | 49 | 64 |
HSPB8 | 26353 | CTCCTGCCACTACCCAAGC | GGCCAAGAGGCTGTCAAGT | 123 | 64 |
HSPB11 | 51668 | TGATGGCTCCGCTACTTACTT | GCAGAAACGCTATGCACAGAT | 78 | 60 |
EF1α | 1915 | AAGCTGGAAGATGGCCCTAAA | AAGCGACCCAAAGGTGGAT | 116 | 55–62 |
18S | 100008588 | GTAACCCGTTGAACCCCATT | CCATCCAATCGGTAGCG | 150 | 58–65 |