Fig. 6
Cyclosporine alleviates motor deficits in the MPTP mouse model of PD. a Strength was analyzed using the grip test. Data are expressed as mean ± SEM (n = 5-8 per group). ***P < 0.001 compared to Veh. + Veh. group, ## P < 0.01 compared to MPTP + Veh. group (one-way ANOVA). b-c Modifications in gait pattern were examined using the DigiGait apparatus by measuring the stance width (b) and the step angle variance (c) Data are expressed as mean ± SEM (n = 5-8 per group). *P < 0.05 compared to Veh. + Veh. group, # P < 0.05 compared to MPTP + Veh. group (one-way ANOVA). d Striatal and midbrain sections from all experimental groups were stained for TH and TH + Cresyl violet (CV), respectively. Scale bar: 500 μm for striatal sections and 250 μm for midbrain sections. e Quantification of the number of nigral TH+/CV+ dopamine neurons. Data are expressed as mean ± SEM (n = 5-8 per group) ***P < 0.001 compared to Veh. + Veh. group (one-way ANOVA). f Optical densitometry analysis of striatal TH+ fibers. Data are expressed as mean ± SEM (n = 5-8 per group) ** P < 0.01 compared to Veh. + Veh. group (one-way ANOVA). g-h Western blot analysis of TH (striatum), GFAP (striatum), NFATc3 (midbrain), GLT-1 (striatum), cytochrome c (midbrain), SGC10 (striatum) expression levels. Quantification for each marker is presented as mean ± SEM (n = 5-8 per group) (H1-H5). *P < 0.05 and **P < 0.01 compared to Veh. + Veh. group, # P < 0.05 and ## P < 0.01 compared to MPTP + Veh. group (one-way ANOVA) (a.u: arbitrary units)