Fig. 4

Cyclosporin treatment alleviates motor features in a novel α-synuclein-based model of PD. a Motor function was examined using the hanging wire test in AAV-FlexOFF-α-synuclein vector injected mice treated with vehicle solution or CsA (i.p., 20 mg/kg, 5 days/week). Data are expressed as mean ± SEM (n = 15 per group). *P < 0.05 (t-test). Tam: tamoxifen. b Striatal and midbrain sections stained for TH. Scale bar: 1 mm. Asterisk on midbrain sections indicates the site of injections of the AAV vector. c Stereological estimation of the number of TH-positive nigral neurons. Data are expressed as mean ± SEM (n = 10 per group). d Western blot analysis of the striatal levels of human α-synuclein and TH levels in both experimental groups. Data are expressed as mean ± SEM (n = 5 per group). *P < 0.05, compared respective vehicle group (t-test)