Fig. 6
From: Differential expression of glucose-metabolizing enzymes in multiple sclerosis lesions
Axonal αKGDH expression, regulation and activity in MS lesions. High magnification immunofluorescence staining of αKGDH (red), porin (green) and SMI312 (magenta) (a-c). The quantitative data are shown in (d). Double labelling of synaptophysin (e,g, red) and αKGDH (f, h, green). The total area stained for αKGDH was determined in synaptophysin-positive and synaptophysin-negative images (i). NAD+-dependent αKGDH activity was reduced in inactive lesions (k) as compared to NAWM (j). The quantitative data are shown in (l). Culture of human neuroblastoma cells under hypoxia or in the presence of ROS or TNF-α and IFN-γ reduced αKGDH mRNA expression (m). *P < 0.05, **P < 0.01