Table 1 Antibodies used for immunocytochemistry
From: Autoimmune encephalitis in humans: how closely does it reflect multiple sclerosis ?
Primary antibody | Antibody type | Target | Source | Staining |
|---|---|---|---|---|
PLP | Mouse (mAB) | Proteolipid protein | MCA8394; AbD Serotec | 1:1000; E |
MAG | Mouse (mAB) | Myelin-associated glycoprotein | ab89780; Abcam | 1:1000; E |
MOG | Mouse /mAB) | Myelin oligodendrocyte glycoprotein | 8-18C5; C. Lininton, Cardiff, UK | 1:1000; C |
GFAP | Mouse (mAB) | Glial fibrillary acid protein | 0410080; ThermoSc | 1:200; E |
pNF | Mouse (mAB) | Phosphorylated Neurofilament | Affinity, SMI31,NA1219, Exeter, UK | 1:20000; E |
APP | Mouse (mAB) | Amyloid precursor protein | MAB348; Chemicon, Temecula, CA, USA | 1:1000; C |
Iba1 | Rabbit (pAB) | Ionized calcium binding adaptor molecule 1 | 019-19741; WAKO Chemicals, Neuss, Germany | 1:3000; E |
CD68 | Mouse (mAB) | Cluster of Differentiation 68 | M0814; Dako | 1:100; E |
p22phox | Rabbit (pAB) | NADPH oxidase | Sc-20781; Santa Cruz, | 1:100; C |
CD3 | Rabbit (mAB) | T-cells | RM-9107-S; Neomarkers, Fremont, CA, USA | 1:2000; E |
CD4 | Mouse (mAB) | CD4 T-cells | ACRIS, 1F6; DM-119-05, San Diego, CA, USA | 1:1000; E |
CD8 | Mouse (mAB) | CD8 T-cells | M7103; Dako, Glostrup, Denmark | 1:250; E |
CD20 | Mouse (mAB) | B-cells | MS-340-S; Neomarkers, Fremont, CA, USA | 1:100; E |
CD138 | Mouse (nAB) | Plasma Cells | Serotec, MCA 681H; UK | 1:500; E |
GranB | Mouse (mAB) | Granzyme B | MS-1157-S; Neomarkers, Fremont, CA, USA | 1:1000; E |
IgG | Rabbit (pAB) | Imunoglobulin G | A0423, DAKO, Glostrup, DK | 1:400; Prot. |
IgA | Rabbit (pAB) | Immunoglobulin A | A042, DAKO, Glostrup, DK | 1:2000; Prot. |
IgM | Rabbit (pAB) | Immunoglobulin M | A426; DAKO, Glostrup, DK | 1:400; Prot |
C9neo | Rabbit (pAB) | Compement C9neo antigen | P. Morgan; Cardiff, UK | 1:2000; Prot |
PCNA | Mouse (mAB) | Proliferating cell nuclear antigen | M0879, DAKO, Glostrup, DK | 1:10000; C |
E06 | Mouse (mAB) | Oxidized phospholipids | Avantilipids; 330001S, Alabaster, AL, USA | 10ÎĽg/ml; none |
α-Syn | Mouse (mAB) | α-Synuclein | Aj.ROBOSCREEN, mab 5G4 | 1:1000; C |
AT8 | Mouse (mAB) | Phosphorylated tau-protein (PHF) | Immunogenetics, Ghent, Belgium | 1:2000; C |
AIF | Rabbit (pAB) | Apoptosis inducing factor | Milipore, AB16501; Temecula, CA | 1:250; C |