Fig. 5

Phenotypic distribution of ATF3-expressing DRG neurons after TAM treatment. a NF200, expressed by large diameter mechanosensory DRG neurons. Arrowhead indicates a few NF200-negative profiles. b CGRP, expressed by peptidergic nociceptors. c IB4 lectin binding by non-peptidergic nociceptors, doubly-labeled cells are indicated by arrows. d TH expression by small diameter DRG neurons. e Quantification of marker expression by tdtomato-positive neurons. The vast majority of recombined DRG neurons express NF200. f Trigeminal mesencephalic proprioceptive neurons also undergo recombination with TAM treatment. g Central projections of recombined neurons are most dense in the ascending dorsal columns and in Clarke’s column (arrow). Other recombined structures are vascular endothelial and smooth muscle cells and ependymal cells (see also Fig. 2). All images are single confocal planes