Fig. 3

Characterization of recombined cells in the dentate olfactory bulb and dentate gyrus. a Olfactory bulb. recombined cells are neuronal, as is evident from morphology and colocalization with NeuN (arrows). b Dentate gyrus. Here too, tdtomato-expressing cells express NeuN and the dentate granule cell lineage marker calbindin D28k (Calb D28k). Asterisks indicate recombined endothelial cells. c Recombined neurons do not undergo cell death. Very few cells in either the olfactory bulb or dentate gyrus expressed the pro-apoptotic marker activated caspase 3 (aCasp3) or had condensed chromatin, and these were never tdtomato-positive. Shown is an example of a rare dying neuron from the dentate gyrus