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Fig. 1 | Acta Neuropathologica Communications

Fig. 1

From: Tamoxifen induces cellular stress in the nervous system by inhibiting cholesterol synthesis

Fig. 1

Characterization the naïve ATF3-CreERT2:stopfl/fltdtomato mouse. a Genetic strategy used to generate the ATF3 CreERT2 mouse. The wild-type ATF3 locus (ATF3 wt) was modified to generate a transgenic construct (ATF3 Cre + neo), in which CreERT2 was inserted immediately after the ATG start codon. Numbers in brackets designate length of fragments in kilobases. The construct was electroporated into 129SvEv mouse embryonic stem cells and positive clones were selected via neomycin (neo) and identified using Southern blots: three clones are shown as an example. Positive founders were bred with a flp-expressing line to remove the neomycin cassette, and the final ATF3 Cre mice were crossed with a ROSA-tdtomato line to yield an ATF3CreERT2 reporter mouse (ATF3 Cre x tdtomato). Schematics are not drawn to scale. b Terminals of trigeminal (left) and DRG axons (right) in the brainstem and upper cervical cord, respectively (regions in boxes enlarged in b’ and b”). The arrow indicates sensory axons in the dorsal funiculus. All images are 30 μm-thick confocal orthogonal projections. c A tdtomato-positive sensory neuron from a cervical DRG (42 μm-thick z-projection). d Rare recombined granular neurons from the dentate gyrus (left) and olfactory bulb (right) (30 μm-thick z-projections). e Occasional clusters (probably clonal) of microglia (left, here from the cerebellum) and vascular endothelial cells (right, in this case from the midbrain) (30 μm-thick z-projections)

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