Fig. 9

Thioflavin T binding assay detects abnormal alpha-synuclein in the brains of mice injected with brain extracts from MSA and iLBD patients. Shown is the time dependent increase in Thioflavin T (ThT) fluorescence measured in relative fluorescence units (RFU) over a period of 72 h. Brain homogenates from mice injected with PBS did not seed aggregation of alpha-synuclein, suggesting that they did not contain abnormal alpha-synuclein. In contrast, brain homogenates from mice injected with brain extracts from patients with MSA1, MSA2, iLBD1, and iLBD2 lead to an increase in ThT fluorescence over time, suggesting that the brains of these mice contained abnormal alpha-synuclein that was seeding competent. Each row represents replicates from the same whole brain homogenate of mice collected at 9 months post injection