Skip to main content
Fig. 3 | Acta Neuropathologica Communications

Fig. 3

From: Murine Aβ over-production produces diffuse and compact Alzheimer-type amyloid deposits

Fig. 3

Comparison of APP and PS1 expression levels in PrP.MoAβ/PS1 and PrP.HuAβ/PS1 mice. The brains of 2 non-transgenic (age 1.6–2.4 months) and 3 each PrP.MoAβ/PS1 (Line D-943; aged 2.5–3.3 months) and PrP.HuAβ/PS1 (Line 85, aged 1.6–2.4 months) mice were homogenized in RIPA buffer, the supernatants were analyzed by immunoblotting 4–20% SDS-PAGE gels with antibodies to PS1 (1:5000, top row), human APP (mAb 6E10, 1:1500, second row), total APP (mAb 22C11, 1:1000, third row), or mouse SOD1 (1:2500, fourth row). The mouse SOD1 immunoblot served as a loading control. The levels of PS1dE9 and total APP were similar in the two lines of mice. Each lane contains 50 μg of total protein

Back to article page
\