Fig. 2

Analysis of Aβ peptides produced by mouse N2a cells transiently expressing mouse and MoHu chimeric APP genes. a Examples of mass spectrometry data from the analysis of cell culture medium of mouse N2a cells transiently transfected with expression plasmids encoding moAPP-WT, moAPPswe, MoHuAPP-WT, and MoHuAPPswe. The Aβ in the medium was immunoprecipitated with the monoclonal antibody 4G8 and then analyzed by mass spectrometry. The positions of mouse and human Aβ40 (mAβ40 and hAβ40) are noted on the spectrum traces. b Analysis of data from repeated experiments (n = 3) demonstrated that the Swedish mutations in mouse APP shift cleavage to produce a greater amount of Aβ1-40 over Aβ11-40. Standard deviation (SD) was shown at the error bars