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Table 1 Bioassay of rPrP amyloid fibrils

From: Two alternative pathways for generating transmissible prion disease de novo

Inoculum ns/nt a nPKres/nt b Euthanized, days post inocul.
F0.5 rPrP fibrils (1st passage) 0/7 6/7 723
F0.5 fibril-induced atypical PrPres (1st passage) 1/10 10/10 2 at 441, 582, 2 at 630, 5 at 636
Brain-derived atypical PrPres amplified in dgPMCA 0/4 4/4 606
atypical PrPres from S05 brainc 0/4 4/4 606
BH from animal inoculated with F0.5 fibril-induced atypical PrPres (2nd passage) 4/6 6/6 630
  1. a number of animals with clinical signs over the total number of animals survived to the end of the experiment
  2. b number of animals with PK-resistant PrP in BHs detectible by Western blot over the total number of animals survived to the end of the experiment
  3. c 0.3 % brain material from the second passage of SO5 [11]