Two alternative pathways for generating transmissible prion disease de novo

Table 1 Bioassay of rPrP amyloid fibrils

Inoculum	n_s/n_t ^a	n_PKres/n_t ^b	Euthanized, days post inocul.
F^0.5 rPrP fibrils (1st passage)	0/7	6/7	723
F^0.5 fibril-induced atypical PrPres (1st passage)	1/10	10/10	2 at 441, 582, 2 at 630, 5 at 636
Brain-derived atypical PrPres amplified in dgPMCA	0/4	4/4	606
atypical PrPres from S05 brain^c	0/4	4/4	606
BH from animal inoculated with F^0.5 fibril-induced atypical PrPres (2nd passage)	4/6	6/6	630

^a number of animals with clinical signs over the total number of animals survived to the end of the experiment
^b number of animals with PK-resistant PrP in BHs detectible by Western blot over the total number of animals survived to the end of the experiment
^c 0.3 % brain material from the second passage of SO5 [11]

ISSN: 2051-5960