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Fig. 4 | Acta Neuropathologica Communications

Fig. 4

From: Two alternative pathways for generating transmissible prion disease de novo

Fig. 4

2D analysis of sialylation status of PrPSc and atypical PrPres. 2D analysis of charge distribution of S05 brain-derived PrPSc (a) and atypical PrPres from animals inoculated with F0.5 fibrils (b, upper panel), atypical PrPres induced in animals by inoculating brain-derived atypical PrPres amplified in dgPMCAb (b, middle panel), or asymptomatic S05 animals that lacked PrPSc (b, lower panel). Black triangles, white triangles and arrows mark di-, mono-, and unglycosylated glycoforms, respectively. The distributions of all three glycoforms of atypical PrPres are shifted toward acidic pH relative to those of PrPSc due to differences in number of charged amino acid residues and, accordingly, pIs between atypical PrPres and PrPSc. All samples were treated with PK. Western blots were stained with 3F4 (a) and SAF-84 (b)

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