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Fig. 5 | Acta Neuropathologica Communications

Fig. 5

From: The A673T mutation in the amyloid precursor protein reduces the production of β-amyloid protein from its β-carboxyl terminal fragment in cells

Fig. 5

CHAPSO-solubilized γ-secretase assay. Recombinant C99-FLAG substrates were incubated with solubilized γ-secretase and subjected to Western Blotting to visualize and quantify levels of Aβ production (a). Aβ production from C99 A2T was indistinctive to that from C99 WT. Data represent means ± SD of three independent experiments. NS, not significant (unpaired t-test). Substrates were immobilized on anti-FLAG magnetic beads and incubated with CHAPSO-solubilized γ-secretase (b). After sufficient wash, the beads were subjected to Western Blotting. γ-Secretase interacted with C99 A2T as well as C99 WT. -, DMSO vehicle control; D, 1 μM DAPT. Quantitative analyses of interacted γ-secretase components (c). Data represent means ± SD of three independent experiments in the absence of 1 μM DAPT. NS, not significant (ANOVA, Scheffe’s post hoc test compared with WT DMSO)

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Acta Neuropathologica Communications

ISSN: 2051-5960

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