Fig. 4

Conditioned media of C99 WT and C99 A2T cells were subjected to Western blotting to visualize and quantify the Aβ and p3 species. C99 A2T cells exhibited reduced levels of Aβ40 and Aβ42, but not of p3 40 (a). Lysates of transfectants were subjected to Western blotting, to visualize and quantify GFP, C99, and intracellular Aβ (b). The lysate of C99 WT cells was loaded into a gel, as indicated by standard curve. The levels of GFP and C99 in cells expressing C99 WT and C99 A2T were indistinctive, whereas intracellular Aβ levels were significantly reduced in C99 A2T cells. Data represent means ± SD of three independent experiments (c). NS, not significant. * P < 0.05 (unpaired t-test)