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Fig. 2 | Acta Neuropathologica Communications

Fig. 2

From: The A673T mutation in the amyloid precursor protein reduces the production of β-amyloid protein from its β-carboxyl terminal fragment in cells

Fig. 2

Pulse chase analysis of APP processing. Schematic diagram of pulse chase analysis of APP processing (a). CHO cells were transfected with APP WT or APP A673T construct and treated with 1 μg/ml tetracycline for 4 h. After replacing media, cells and media were collected at the time indicated. Detection of APP, sAPPβ, C99, Aβ, C83 and sAPPα by Western Blotting (b). C99 was barely detected by using anti-FLAG antibody, although robust C83 bands were visualized. Quantitative analyses of APP, sAPPβ, C99, Aβ, C83 and sAPPα (c). Levels of sAPPβ and Aβ were reduced in APP A673T cells compared with those in APP WT cells, while levels of APP and C99 were almost similar to those of APP WT cells. It is interesting to note that generation rate of C83 is distinct from those of APP and C99. Data represent means ± SD of three independent experiments. Open circle, WT; closed circle, A673T. *P < 0.05; **P < 0.01 (unpaired t-test)

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Acta Neuropathologica Communications

ISSN: 2051-5960

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