Fig. 3

Analysis of Flnc expression in Grn knockout mice at transcript and protein level. qRT-PCR analysis of the long and short isoform of Flnc (a) measured in progranulin knockout mice of different ages. We analyzed the expression levels of both long and short isoforms of mouse Flnc in heterozygous Grn^+/- and homozygous Grn^-/- mice and wild-type (Wt) animals of 3 months (n = 4), 9 months (n = 4), 16-18 months (n = 6) and 24 months (n = 5) of age. b Increased FlnC expression levels were confirmed on protein level using quantitative immunoblot analysis. Two protein bands are detected around the height of mouse FLNC using the Kinasource AB152 anti FLNC antibody. The upper band is FLNC specific as determined by Western blotting using lysates from FlnC knockout mice (data not shown). The lower band is therefore considered as an aspecific protein band. *P < 0.05; ***P < 0.001, n.s. not significant